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学科：基础兽医学 电话/传真：13509713780 电子邮件：fanglx@scau.edu.cn 通讯地址：广东省广州市天河区五山路华南农业大学483号兽医学院科技楼
方亮星，女，1987年出生于湖北省黄冈市，农学博士，从事动物源细菌耐药性及防控策略研究。自2011年以来一直围绕动物源细菌耐药性开展研究工作，基于大量的耐药性调查和耐药基因的传播特征分析，发现优势流行质粒IncHI2、IncFII、IncX4 等是介导重要耐药基因（blaCTX-M、oqxAB、mcr、fosA3、rmtB等）快速传播的重要载体，一定程度上解释了我国动物源沙门菌和大肠杆菌等多重耐药甚至泛耐药广泛出现的原因。进一步基于质粒-宿主菌实验室进化模型，发现缓解携带多重耐药质粒的适应性代价是质粒的适应性与其介导的耐药性之间权衡；还进一步发现质粒携带的多重耐药区的缺失和染色体arcA失活突变共同导致了多重耐药IncHI2质粒与鼠伤寒沙门菌在不同的药物选择压力下的共同适应和共同进化，为多重耐药质粒在细菌内广泛而稳定存在的机制提供一定的见解，为药物尤其是联合药物压力下多重耐药质粒介导的耐药性演变及防控细菌耐药性提供一定的参考。此外，还开展抗菌肽对耐药病原菌的抗菌活性及机制研究。目前，主要围绕携带多重耐药质粒对宿主沙门菌的适应性代价机制及其在宿主菌中稳定维持的分子机制、以及抗菌肽对抗多重耐药菌的抗菌活性及作用机制开展研究。已获得2项国家自然科学基金项目，其中1项已顺利结题，主持2项十四五国家重点研发计划项目子课题，获得1项广州市科技计划项目1项并顺利结题。此外，参与细菌耐药性研究相关的多项国家级研究项目，包括十三五国家重点研发计划、国家自然科学基金重点国际（地区）合作与交流项目、国家自然科学基金重点项目等。以第一作者（含共一）或通讯作者在mSystems、J Antimicrob Chemother、Antimicrob Agents Chemother、Appl Environ Microbiol、Vet Microbiol等国际期刊上发表SCI论文20余篇。
2011/09-2017/06，华南农业大学，兽医学院基础兽医学系，博士，导师：刘雅红
2016/05-2017/05，爱荷华州立大学(美国)，兽医学院微生物与预防兽医学系，联合培养博士生，导师：张启敬/刘雅红
2006/09-2011/06，华南农业大学，兽医学院基础兽医学系，学士
2021/1-至今：华南农业大学，兽医学院，基础兽医学，副研究员
2017/7-2020/12：华南农业大学，兽医学院，基础兽医学，首聘副研究员
动物源细菌耐药性及防控策略
1.主持国家自然科学基金面上项目（基金号32373063），ArcA调控TCA循环和氧化应激介导多重耐药IncHI2质粒与鼠伤寒沙门菌补偿性进化的机制研究，2024.01-2027.12 （经费50万）
2.主持 “十四五”国家重点研发计划子课题（基金号2023YFD1801001-02），畜禽源沙门菌的传播动力学研究及病原和组学数据库构建，2023.12-2027.12 （经费50万）
3.主持 “十四五”国家重点研发计划子课题（基金号2022YFD1800404-02），影响耐药病原菌在畜禽肠道定植的关键基因，2022.11-2025.12 （经费50万）
4.主持国家自然科学基金青年基金项目（基金号31802244），多重耐药杂合型IncHI2质粒特征及抗生素压力对其形成的影响研究，2019.01-2021.12 （经费26万）
5.主持广州市科技计划项目，沙门菌携带多重耐药IncHI2质粒适应性代价的分子机制，2022-04-2024.03（经费5万）
1. Ming Cheng #, Jing-Jing Dai #, Jin-Fei Zhang, Yu-Ting Su, Si-Qi Guo, Ruan-Yang Sun, Dong Wang, Jian Sun, Xiao-Ping Liao, Sheng Chen, Liang-Xing Fang. Evolution and maintenance of a large multidrug-resistant plasmid in a Salmonella enterica Typhimurium host under differing antibiotic selection pressures. mSystems. 2024 Nov 19;9(11):e0119724.
2. Qian Li, Jing-Jing Dai, Shu-Yi Chen, Ruan-Yang Sun, Dong Wang, Shuan-Cheng Bai, Min-Ge Wang, Jian Sun, Xiao-Ping Liao, Ya-Hong Liu, Liang-Xing Fang. Prevalence and molecular characteristics of intestinal pathogenic Escherichia coli isolated from diarrheal pigs in Southern China. Vet Microbiol. 2024 Sep:296:110171.
3. Ruan-Yang Sun #, Liang-Xing Fang #, Bi-Xia Ke, Jian Sun, Zuo-Wei Wu, You-Jun Feng, Ya-Hong Liu, Chang-Wen Ke, Xiao-Ping Liao. Carriage and Transmission of mcr-1 in Salmonella Typhimurium and Its Monophasic 1,4,[5],12:i:- Variants from Diarrheal Outpatients: a 10-Year Genomic Epidemiology in Guangdong, Southern China. Microbiol Spectr[J]. 2023 Feb 14;11(1):e0311922.
4. Wen-Ying Guo#, Hui Zhang#, Ming Cheng, Min-Rui Huang, Qian Li, Yu-Wei Jiang, Ji-Xing Zhang, Ruan-Yang Sun, Min-Ge Wang, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun*, Liang-Xing Fang*. Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China. Microbiol Spectr[J]. 2022 Aug 15;e0250321.
5. Ruan Yang Sun#, Wen Ying Guo#, Ji Xing Zhang, Min Ge Wang, Lin Lin Wang, Xin Lei Lian, Bi Xia Ke, Jian Sun, Chang Wen Ke, Ya Hong Liu, Xiao Ping Liao, Liang Xing Fang*. Phylogenomic analysis of Salmonella Indiana ST17, an emerging MDR clonal group in China. J Antimicrob Chemother[J]. 2022 Jul 26;dkac243.
6. Jin-Fei Zhang#, Liang-Xing Fang#, Man-Xia Chang, Ming Cheng, Hui Zhang, Teng-Fei Long, Qian Li, Xin-Lei Lian, Jian Sun*, Xiao-Ping Liao*, Ya-Hong Liu*. A Trade-Off for Maintenance of Multidrug-Resistant IncHI2 Plasmids in Salmonella enterica Serovar Typhimurium through Adaptive Evolution. mSystems [J]. 2022 Oct 26;7(5):e0024822.
7. Dao-Ji Cen#, Ruan-Yang Sun#, Jia-Lin Mai, Yu-Wei Jiang, Dong Wang, Wen-Ying Guo, Qi Jiang, Hui Zhang, Jin-Fei Zhang, Rong-Min Zhang, Jian Sun, Xiao-Ping Liao, Ya-Hong Liu*, Liang-Xing Fang*. Occurrence and Transmission of blaNDM-Carrying Enterobacteriaceae from Geese and the Surrounding Environment on a Commercial Goose Farm. Appl Environ Microbiol [J]. 2021 Apr 27;87(10):e00087-21.
8. Ke Cheng#, Liang-Xing Fang#, Qian-Wen Ge, Dong Wang, Bing He, Jia-Qi Lu, Zi-Xing Zhong, Xi-Ran Wang, Yang Yu, Xin-Lei Lian, Xiao-Ping Liao, Jian Sun*, Ya-Hong Liu*. Emergence of fosA3 and blaCTX-M-14 in Multidrug-Resistant Citrobacter freundii Isolates From Flowers and the Retail Environment in China. Front Microbiol. 2021 Feb 5;12:586504.
9. Liang-Xing Fang#, Chong Chen#, Chao-Yue Cui, Xing-Ping Li, Yan Zhang, Xiao-Ping Liao, Jian Sun*, Ya-Hong Liu*. Emerging High-Level Tigecycline Resistance: Novel Tetracycline Destructases Spread via the Mobile Tet(X) [J]. Bioessays.2020. 42(8):e2000014.
10. Liang-Xing Fang, Qi Jiang, Guo-Hui Deng, Bing He, Ruan-Yang Sun, Jin-Fei Zhang, Dao-Ji Cen, Yuan-Yuan Miao, Dong Wang, Wen-Ying Guo, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao*. Diverse and flexible transmission of fosA3 associated with heterogeneous MDR regions in Salmonella enterica serovar Typhimurium and Indiana isolates [J]. Antimicrob Agents Chemother. 2020;64(2):e02001-19.
11. Ruan-Yang Sun#, Bi-Xia Ke#, Liang-Xing Fang#, Wen-Ying Guo, Xing-Ping Li, Yang Yu, Si-Lin Zheng, Yu-Wei Jiang, Dong-Mei He, Jian Sun, Chang-Wen Ke, Ya-Hong Liu, Xiao-Ping Liao*. Global clonal spread of mcr-3-carrying MDR ST34 Salmonella enterica serotype Typhimurium and monophasic 1,4,[5],12:i:- variants from clinical isolates [J]. J Antimicrob Chemother. 2020. 75:1756-1765.
12. Liang-Xing Fang#, Chong Chen#, Dong-ling Yu#, Run-Shi Yang, Chao-Yue Cui, Liang Chen, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun*. Complete Nucleotide Sequence of a Novel Plasmid Bearing the High-Level Tigecycline Resistance Gene, tet(X4) [J]. Antimicrobial Agents and Chemotherapy, 2019;63(11):e01373-19.
13. Liang-Xing Fang#, Guo-Hui Deng#, Qi Jiang, Dao-Ji Cen, Run-Shi Yang, Yun-Yun Feng, Jing Xia, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao*. Clonal expansion and horizontal transmission of epidemic F2:A1:B1 plasmids involved in co-spread of rmtB with qepA and blaCTX-M-27 in extensively drug-resistant Salmonella enterica serovar Indiana isolates [J]. Journal of Antimicrobial Chemotherapy, 2019;74(2):334-341.
14. Liang-Xing Fang#, Xing-Ping Li#, Liang Li, Mu-Ya Chen, Cai-Yan Wu, Lu-Lu Li, Xiaoping-Liao, Ya-hong Liu, Jian Sun*. ISEcp1-mediated transposition of chromosome-borne blaCMY-2 into an endogenous ColE1-like plasmid in Escherichia coli [J]. Infection and Drug Resistance, 2018; 11: 995-1005.
15. Liang-Xing Fang, Jia-Hong Duan, Mu-Ya Chen, Hui Deng, Hua-Qing Liang, Yan Q. Xiong, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao*. Prevalence of cfr in Enterococcus faecalis strains isolated from swine farms in China: Predominated cfr-carrying pCPPF5-like plasmids conferring "non-linezolid resistance" phenotype [J]. Infection, Genetics and Evolution, 2018, 62(4)：e02068-17.
16. Liang-Xing Fang, Xing-Ping Li, Guo-Hui Deng, Shu-Min Li, Run-Shi Yang, Zuo-Wei Wu, Xiao-Ping Liao, Jian Sun*, Ya-Hong Liu*. High genetic plasticity in multidrug resistant ST3-IncHI2 plasmids revealed by sequence comparison and phylogenetic analysis [J]. Antimicrobial Agents and Chemotherapy, 2018，62(4)：e02068-17.
17. Jian Sun#, Liang-Xing Fang#, Zuowei Wu, Hui Deng, Run-Shi Yang, Xing-Ping Li,Shu-Min Li, Xiao-Ping Liao, Youjun Feng, Ya-Hong Liu*. Genetic analysis of the IncX4 plasmids: Implications for a unique pattern in the mcr-1 acquisition [J]. Scientific Reports, 2017, 7(1).
18. Liangxing Fang, Xingping Li, Liang Li, Shumin Li, Xiaoping Liao, Jian Sun* and Yahong Liu*. Co-spread of metal and antibiotic resistance within ST3-IncHI2 plasmids from E. coli isolates of food-producing animals [J]. Scientific Reports, 2016, 6: 25312.
19. Xiao-Ping Liao#, Liang-Xing Fang#, Liang Li, Jian Sun, Xing-Ping Li, Mu-Ya Chen, Hui Deng, Qiu-E Yang, Xue Li, Ya-Hong Liu*. Characterization of Chromosomal qnrB and ampC Alleles in Citrobacter Freundii Isolates from Different Origins [J]. Infection, Genetics and Evolution, 2015, 35: 214-20.
20. Liang-Xing Fang, Jian Sun, Liang Li, Hui Deng, Ting Huang, Qiu-E. Yang, Xue Li, Mu-Ya Chen, Xiao-Ping Liao, Ya-Hong Liu*. Dissemination of the chromosomally encoded CMY-2 cephalosporinase gene in Escherichia coli isolated from animals. International Journal of Antimicrobial Agents [J]. 2015, 46(2): 209-13.